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SRX7405182: TBM3131:mini-exon-seq
1 ILLUMINA (Illumina MiSeq) run: 250,258 spots, 150.7M bases, 88.8Mb downloads

Design: 1) Mini-exon PCR following Fernandes et al. 2001 (Trans R Soc Trop Med Hyg), 2) Gel-based purification (PureLink Quick Gel Extraction Kit, Thermo Fisher Scientific), 3) Index PCR, 4) Bead-based purification (Agencourt AMPure XP, Beckman Coulter), 5) Equimolar pooling, 6) 600-cycle Illumina MiSeq (Reagent Kit v3)
Submitted by: Pontifical Catholic University of Ecuador
Study: Trypanosoma cruzi and T. rangeli mini-exon sequencing
PRJNA596271 • SRP238060 • All experiments • All runs
show Abstracthide Abstract
Mini-exon fragments were amplified from T. cruzi and/or T. rangeli infected triatomine intestinal content by polymerase chain reaction and sequenced on the Illumina MiSeq platform. Triatomines (Rhodnius ecuadoriensis and Panstrongylus chinai) were collected in Loja Province, southern Ecuador. Sequencing also included amplicons generated from T. cruzi reference clone epimastigote DNA.
Sample:
SAMN13616563 • SRS5852756 • All experiments • All runs
Organism: Trypanosoma sp. TBM3131
Library:
Name: ME_TBM3131
Instrument: Illumina MiSeq
Strategy: AMPLICON
Source: METAGENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 250,258 spots, 150.7M bases, 88.8Mb
Run# of Spots# of BasesSizePublished
SRR10729030250,258150.7M88.8Mb2020-05-01

ID:
9691658

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